dna amplification meaning in English
dna 扩增
dna扩增
Examples
- Polymerase chain reaction is a rapidly developing and widely used dna amplification technique , which is widely applied in life science and other related fields
聚合酶链式反应( polymerasechainreaction ,简称pcr )技术是发展很快、应用很广的体外扩增基因片断技术,在生命科学研究及诸多相关领域已经得到了广泛应用。 - Many streptomyces species exhibit a very high degree of genetic instability which is usually manifested as genomic rearrangement such as large deletion , and high - level dna amplification of those sequences flanking the deletion
许多链霉菌表现出高度的遗传不稳定性,通常为大片段缺失或基因组重排,以及缺失区域两侧序列的高水平dna扩增。 - In chapter three , genomic dna were extracted from four individuals from sinotubimorpha porracea and grateloupia filicina , respectively and from g . livida as a control . sixties primers were used for dna amplification and a total of 120 amplification products range from 250bp to 2500bp were generated . distance matrix and dendrograms were obtained as described in last paragraph
在第三部分,应用ctab法提取了管形藻、蜈蚣藻各4个个体及作为对照的舌状蜈蚣藻的基因组dna , 60个引物的rapd分析获得120个片段大小在250 - 2500bp之间的扩增片段,遗传距离和聚类分析同前。 - Pcr - based analysis method was carried out in the following study . dna amplification by pcr in cgg repeats were performed on 154 people as normal control ( 73 males and 81 females ) and 23 members from four x - linked mental retardation ( xlmr ) families . after electrophoresed on a 6 % - denaturing polyacrylamide gel ( acr : bis = 19 : l ) , genotypes of the objects were determined by analyzing pcr products
本研究采用较以往更为简便有效的pcr技术方法,对随机抽取的154人的正常群体(男性73人、女性sl人)及来自四个智力低下家系的23名成员进行了fmr基因cgg重复序列的扩增,利用变性聚丙烯酸胺凝胶电泳技术对不同等位基因类型进行分离和统计。 - Sixties primers were used for dna amplification and a total of 237 amplification products range from 450bp to 2500bp were generated . a similarity matrix for all pairwise comparisons was calculated using nei and li ' s formula and then transformed to distance matrix . dendrograms were constructed by applying unweighted pair - group arithmetic average ( upgma ) and neighboring - jointing cluster analyses using the phylip software
在第二部分,应用改进的ctab法提取了石莼属和浒苔属各3个种及作为对照的刚毛藻的基因组dna , 60个引物被用于扩增,共获得237个片段大小在450 - 2500bp之间的扩增片段,依据nei和li ( 1979 )的公式计算出样品成对比较的相似性距阵并换算成遗传距离,应用phylip软件包,按照upgma法和n - j法分别构建聚类图。